Change-O is a collection of tools for analyzing immunoglobulin sequences.

Dramatic improvements in high-throughput sequencing technologies now enable large-scale characterization of immunoglobulin (Ig) repertoires, defined as the collection of trans-membrane antigen-receptor proteins located on the surface of T and B lymphocytes. Change-O is a suite of utilities to handle advanced analysis of Ig sequences following germline segment assignment. Change-O handles output from IMGT/High V-quest and works off of a tab-delimited database file. It includes features for creating a personalized genotype, identifying sequences that are from a single B cell clone and inferring its lineage tree, analyzing amino acid properties, calculating diversity, generating a model of somatic hypermutation, and quantifying selection pressure. Record sorting, grouping, and sampling operations are also included.

Change-O Utilities


Change-O: a toolkit for analyzing large-scale B cell immunoglobulin repertoire sequencing data.
Gupta NT*, Vander Heiden JA*, Uduman M, Gadala-Maria D, Yaari G, Kleinstein SH.
Bioinformatics 2015; doi: 10.1093/bioinformatics/btv359

Additional Ig Repertoire Tools & Component Pages